用于乏氧监测的红色荧光探针是一种全新设计的高性能探针，用于实时测定活细胞的缺氧状态。活细胞在含正常氧气浓度（21%）的环境中，荧光非常弱，基本不发出荧光；而当氧水平降低到低于5%时，会发出明亮的红色荧光；细胞的缺氧程度越高，探针的荧光越强。该红色乏氧监测试剂具有完全可逆的响应，当细胞中的氧浓度恢复至正常水平时，荧光随之消失。 乏氧Hypoxia红色探针可渗透活细胞，与活细胞孵育仅1个小时后，就可以快速地观测细胞内的乏氧状态。该试剂是检测肿瘤、3D Spheroids、球体、神经元细胞或组织周围乏氧状态的理想工具。 该探针可使用488nm的蓝色激光器进行有效激发，其最大发射波长位于615nm处，且具有锐利的荧光发射峰，因此非常有利于荧光光谱分离，该探针具有可逆的氧气响应行为，可实现细胞内缺氧状态的动态观测。 The red fluorescent probe for intracellular hypoxia monitoring is a newly designed high-performance probe for real-time determination of the anaerobic state of live cells. When the cells were incubated in an environment with normal oxygen concentration (21%), the fluorescence is very weak and almost no fluorescence is emitted; however, when the oxygen level drops below 5%, bright red fluorescence can be directly observed; the higher the degree of anaerobic state of the cell, the stronger the fluorescence of the probe. The red hypoxia monitoring reagent has a fully reversible response, and the fluorescence disappears as the oxygen concentration in the cell returns to normal levels. The hypoxia-sensitive red probe can penetrate living cells and can be used to quickly observe the state of hypoxia within cells after incubating with the probe for just 1 hour. This reagent is an ideal tool for detecting hypoxia in tumors, 3D Spheroids, spheres, neuronal cells or tissues. This probe can be effectively excited using a 488nm laser, with its maximum emission wavelength at 615nm and a sharp fluorescence emission peak, thus being very beneficial for fluorescence spectroscopy separation. The probe has fully reversible oxygen response behavior, allowing for dynamic observation of hypoxic state in cells.