超小尺寸纳米探针，具有优异的生物相容性和在水中的稳定性，在4℃水溶液中避光保存1年以上，没有发现明显的沉降和团聚，产品在出厂前已进行灭菌处理，可直接使用。细胞外OCR试剂具有优异的氧气响应灵敏度（中图），可通过荧光强度和门控荧光寿命测量技术实现细胞外或培养基中氧浓度的连续不间断测量，是进行细胞OCR测量的良好工具。如右图所示，细胞外OCR试剂可根据客户需求，定制不同波长、不同荧光寿命的试剂，避免与您细胞成像的染料之间发生光谱重叠，为您提供定制化的解决方案。 We had developed ultra-small nanoprobe for measuring extracellular oxygen consumption rate (OCR). The reagent has excellent biocompatibility and stability in water. It can be stored in a solution at 4 ℃ for more than 1 year without obvious sedimentation or aggregation. The product has been sterilized before delivery and can be used directly without any treatment. Extracellular OCR reagents have excellent oxygen response sensitivity (as shown in the middle figure), and can achieve continuous measurement of oxygen concentration outside cells or in culture media via fluorescence intensity and time-gated fluorescence lifetime measurement techniques. They are an ideal tool for extracellular OCR measurement. As shown in the right figure, extracellular OCR reagents can be customized with different wavelengths and fluorescence lifetimes, we also offer customized solution according to your special needs in order to avoid spectral overlap with the other cell-staining dyes.

细胞外OCR试剂已经被证明了不会被细胞内吞，可以稳定地悬浮在培养基中。通过连续测量胞外的氧气消耗速率，获得活细胞的线粒体功能状态，如左图所示，通过在培养基中添加药物调控活细胞线粒体功能，可有效地调控细胞的耗氧速率OCR，从而实现无创、不影响细胞正常能量代谢的细胞OCR分析，该试剂测量速度快、准确度高、测量通量大，是进行活细胞线粒体功能评估和能量代谢分析的有力工具。在电穿孔Electroporation技术的帮助下，该试剂可快速扩散到细胞液中，实现细胞质中的氧浓度实时测量。 The extracellular oxygen consumption rate (OCR) reagent has been proven not to be endocytosed by living cells and can be stably suspended in the culture medium for long duration. By continuously measuring the oxygen consumption rate outside the cell, the mitochondrial function state of live cells can be obtained, as shown in the left figure. By regulating the mitochondrial function of live cells in the culture medium by adding mitochondrial inhibitors, the oxygen consumption rate (OCR) of cells can be effectively regulated, thereby achieving non-invasive and non-disruptive cell OCR analysis without affecting the normal energy metabolism of cells. The reagent has a fast response, high accuracy, and high measurement throughput, and is a powerful tool for live cell mitochondrial function assessment and energy metabolism analysis. With the help of Electroporation technology, the reagent can quickly diffuse into the cell fluid and realize real-time measurement of oxygen concentration in the cytoplasm.

细胞外OCR试剂不仅适用于高通量的96孔板进行细胞、药物和培养条件的筛选，也能通过电穿孔Electroporation技术导入到细胞液中，进行细胞液中的显微成像，实现单/活细胞细胞液中氧浓度的测量，对类器官、组织切片也有很好的适用性。这种试剂还可以被喷洒到植物叶片上，进行植物光合作用的分析和研究。 The extracellular OCR reagent is not only suitable for high-throughput 96-well plate screening of cells, drugs, and culture conditions, but can also be useful in studying oxygen concentration in cytoplasm via electroporation. The reagent enables the measurement of oxygen concentration in single/live cells in the extracellular space, in cytoplasm, and is also suitable for organoids, tissue sections. This reagent can also be sprayed onto plant leaves for analysis and research on plant photosynthesis.